Ha, I knew she was just bitter about Akasha!
Moreover, seeing that scene gave me an idea for Issa's motives: Could it be that the reason he would go so far as to even sacrifice the Shuzen clan and help with Alucard's revival is because he's looking for an opportunity to free Akasha from Alucard's body?
Also, Gyokuro's current from is quite familiar, isn't it? *coughwitchhillarccough*