Just a thought here, but... she says "This is the last time."
Doesn't that mean that there has been a first time?
I'm assuming that she's referring to the kiss, here.
Edit:
Ichika considers herself to be out of the race, and is trying to let go of her feelings. She's still willing to push Fuutarou to make a choice, however.
Nino is definitely still in the race, and seems to think that she has a good chance.
Miku is still in the race, absolutely no mistake about that, although she still seems to waver between "Go for it!" and "There's no way our love will bear fruit".
Yotsuba... appears to have decided to drop out of the race as of now, because of a similarly low sense of self-worth that has been Miku's problem the whole time.
Itsuki remains to be seen, but based on previous chapters she may be in the process of falling for Fuutarou, which is pretty late in the game. Who knows how that will play out?
But let's not forget that even though some of the sisters doubt their chances, the choice still rests with Fuutarou. He may well have a different opinion on what they are worthy of.
Edit 2: Called it.