@Ifrittuccia I think Ren’s a girl, they used little sister. Unlike before where they kept using gender neutral words to refer to Ren and translators guessed the gender putting “he”.
@Yopy I think she remembered the chairman and became afraid that the day may come where she’d lose Ren.
I thought Zero had died protecting Yuuki when she was pregnant with Ren... Wasn't that in an early chapter? In any case, I'm glad to see a new chapter!
@Yopy
Basically, Ai was overwhelmed in her emotions and got the huge urge to drink blood. So, she was repeating "No", as to calm herself down from drinking from Ren.